kiran musunuru (Addgene inc)
Addgene inc is a verified supplier 
94
Structured Review
Addgene inc
kiran musunuru
Kiran Musunuru, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kiran+musunuru/pCas9_GFP+(Plasmid+%2344719)/pmc12884776-268-54-58
Average 94 stars, based on 264 article reviews
Kiran Musunuru, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kiran+musunuru/pCas9_GFP+(Plasmid+%2344719)/pmc12884776-268-54-58
Average 94 stars, based on 264 article reviews
kiran musunuru - by Bioz Stars,
2026-09
94/100 stars
Images
Related Articles
Plasmid Preparation:Article Title: AP-2α–Mediated Activation of E2F and EZH2 Drives Melanoma Metastasis Article Snippet: .. The M21 cell line was a gift from the laboratory of Robert Cornell (University of Iowa). pCas9D10A_GFP was a gift from Article Title: Translational reading frame determines the pathogenicity of C-terminal frameshift deletions in MeCP2: an alternative therapeutic approach Article Snippet: .. A separate plasmid, pGuide, a gift from Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Article Snippet: .. The pCas9-mCherry plasmid was generated by replacing the GFP sequence from the pCas9-GFP plasmid with the mCherry sequence. pCas9-GFP was a gift from Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids Article Snippet: .. To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5 , the pGL3 vector with the U6 promoter was used and co‐transfected with pCAS9 into cells. pGL3‐U6‐sgRNA‐PGK‐puromycin was a gift from Xingxu Huang [ ] (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene_51133) and pCas9_GFP was a gift from Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Article Title: In utero adenine base editing corrects multi-organ pathology in a lethal lysosomal storage disease. Article Snippet: Briefly, the CAG promoter driving the SpCas9 to Glu573 fused to the N-terminal Nostoc punctiforme (Npu) DnaE intein was replaced with the CMV chimeric intron promoter from pCI (Promega, Madison, WI) using standard molecular cloning techniques. .. Following the polyadenylation signal, the gRNA cassette—a kind gift from Generated:Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Article Snippet: .. The pCas9-mCherry plasmid was generated by replacing the GFP sequence from the pCas9-GFP plasmid with the mCherry sequence. pCas9-GFP was a gift from Sequencing:Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Article Snippet: .. The pCas9-mCherry plasmid was generated by replacing the GFP sequence from the pCas9-GFP plasmid with the mCherry sequence. pCas9-GFP was a gift from Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Article Title: In utero adenine base editing corrects multi-organ pathology in a lethal lysosomal storage disease. Article Snippet: Briefly, the CAG promoter driving the SpCas9 to Glu573 fused to the N-terminal Nostoc punctiforme (Npu) DnaE intein was replaced with the CMV chimeric intron promoter from pCI (Promega, Madison, WI) using standard molecular cloning techniques. .. Following the polyadenylation signal, the gRNA cassette—a kind gift from Transfection:Article Title: Variant-to-function analysis of the childhood obesity chr12q13 locus implicates rs7132908 as a causal variant within the 3' UTR of FAIM2. Article Snippet: .. 0.5 mg gRNA_Cloning-rs7132908gRNA vector, 0.5 mg pCas9_GFP vector,84 which was a gift from Irradiation:Article Title: Variant-to-function analysis of the childhood obesity chr12q13 locus implicates rs7132908 as a causal variant within the 3' UTR of FAIM2. Article Snippet: .. 0.5 mg gRNA_Cloning-rs7132908gRNA vector, 0.5 mg pCas9_GFP vector,84 which was a gift from HAT Assay:Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids Article Snippet: .. To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5 , the pGL3 vector with the U6 promoter was used and co‐transfected with pCAS9 into cells. pGL3‐U6‐sgRNA‐PGK‐puromycin was a gift from Xingxu Huang [ ] (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene_51133) and pCas9_GFP was a gift from other:Article Title: MYC deregulation sensitizes cancer cells to N-myristoyltransferase inhibition. Article Snippet: Full-length human NDUFAF4 gene was ordered as a 525 bp geneblock from Integrated DNA Technologies. Cell Culture:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Modification:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Mutagenesis:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from CRISPR:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Electroporation:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Polymerase Chain Reaction:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Activity Assay:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Expressing:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from Recombinant:Article Title: Generation of a CRISPR/Cas9-corrected-hiPSC line (DDLABi001-A) from Fabry disease (FD)-derived iPSCs having α-galactosidase (GLA) gene mutation (c.803_806del). Article Snippet: .. Resource Table Unique stem cell line identifier DDLABi001-A Alternative name(s) of stem cell line FD(cor) Institution Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea Contact information of the reported cell line distributor Yong-Mahn Han, ymhan@kaist.ac.kr Type of cell line iPSC Origin human Additional origin info (applicable for human ESC or iPSC) Age: 37 Sex: male Cell Source Fibroblasts Method of reprogramming Retrovirus Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A Cell culture system used mTeSRTM1 medium on matrigel-coated culture dishes Type of Genetic Modification Gene Correction Associated disease OMIM: # 301,500 Fabry disease Gene/locus Gene: GLA Locus: Xq22.1 Mutation: c.803_806del, (p.1268fs*1) Method of modification/site-specific nuclease used CRISPR/Cas9 Site-specific nuclease (SSN) delivery method Electroporation (continued on next column) (continued ) All genetic material introduced into the cells Single guide RNA (sgRNA) and singlestranded oligonucleotide (ssODN) as homology directed repair (HDR) template Analysis of the nuclease-targeted allele status Targeted PCR/DNA sequencing Method of the off-target nuclease activity surveillance Targeted PCR/DNA sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) N/A Inducible/constitutive system details N/A Date archived/stock date February 2020 Cell line repository/bank DDLABi001-A https://hpscreg.eu/ce ll-line/DDLABi001-A Ethical/GMO work approvals The institutional review board of KAIST approved the culture and generation of human iPSCs (approval No.: KH2016-52) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCas9_GFP was a gift from |